1A) Does vesicle budding, vesicle fusion, or both require the input of energy by coupling to favorable reactions?

1A) Does vesicle budding, vesicle fusion, or both require the input of energy by coupling to favorable reactions? Please provide two proteins’ functions involved in vesicle trafficking and their sources of energy.
1B) Does directional transport of cargo into the nucleus (import), out of the nucleus (export), or both require the input of energy by coupling to favorable reactions? Please describe one source of energy for protein transport in the nucleus.
 
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